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After ultracentrifugation at 200,000 g for 20 min at 4 C the resulting supernatants were used in the assays

doi: 10.2478/jomb-2019-0037 36 PanduruNMForsblomCSaraheimoMThornLBierhausAHumpertPMet al

SIRT1 can inactivate mTORC1 in the absence of AMPK (270)

We found low levels of heterogeneity when analysis was done in individual brain regions, However when all areas were combined in the analysis the heterogeneity was much higher (I 2 of 72.18)

Additionally, approximately 17 % of the controls were recruited from hospitals, although it is crucial to emphasise that they were selected from inpatients whose medical conditions were not influenced by dietary modifications

batch 10 (figure 7C) and batch 11 (figure 7D)