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There's really no one-size-fits-all answer, which is why there's so much conflicting evidence

Batch effects, for example, may arise when a subset of samples exhibits abnormalities due to differences in retention times, reagents handling, and so on (171)

[DOI] [PubMed] [Google Scholar] 155.Kumar P., Padi S.S., Naidu P.S., Kumar A

The specific mechanisms by which elevated homocysteine contributes to cardiovascular pathologies and whether interventions to lower homocysteine levels are beneficial remain areas of debate and investigation

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If you are still in the research phase, read straight through